2015 September 29, “Redesigning Recombinase Specificity for Safe Harbor Sites in the Human Genome”, in PLOS ONE, →DOI:
We constructed recombinase libraries by randomly mutagenizing five residues predicted to contact DNA at positions 3–2: Leu 122, Ser 125, Arg 129, Tyr 138 and Gly 139 (Fig 6A ).