Masuku is the former name of Franceville, a city in Gabon. It is also an African surname that may refer to:

• Bandile Masuku, South African politician
• Bongani Masuku, South African vocalist
• Davidson Masuku (1940–2000), South African military commander and physician
• Ernest Masuku (born 1992), Zimbabwean cricketer
• Khethokwakhe Masuku (born 1985), South African association football player
• Lookout Masuku (1940–1986), commander of the Zimbabwe People's Revolutionary Army
• Madala Masuku, South African government official
• Mkhabela Masuku, Zimbabwean born Lawyer and social commentator
• Mario Masuku (1951–2021), Swazi politician
• Menzi Masuku (born 1993), South African football midfielder
• Mkhuphali Masuku (born 1980), Zimbabwean football manager and former player
• Themba N. Masuku, Swazi politician who has served as Deputy Prime Minister of Eswatini since 2018 and as Acting Prime Minister from 2020 to 2021

Masuku is a South African surname usually in the Nguni people Masuku means at night .. It is the 4th popular surname.